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ATCC 33591 atcc 33592 atcc 33593 atcc baa 38 atcc baa 39 atcc baa 40 atcc baa 41 atcc baa 42 atcc baa 43 atcc baa 44 atcc f182 cdc 23
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( A ) Key mitochondrial pathways down-regulated in GSEA performed in patients with MM from the CoMMpass trial (NCT0145429) with poor prognosis (PFS < 2 years, n = 426) versus better prognosis (PFS > 2 years, n = 341). Gene sets for shown pathways are derived from the Reactome database. FDR, false discovery rate. ( B ) Pathways up-regulated in GSEA of RNA-seq data from L363 cells treated with 25 nM IACS for 9 hours compared to vehicle control ( n = 3). KEGG, Kyoto Encyclopedia of Genes and Genomes. ( C ) Volcano plot showing differentially expressed ATF4 targets. ATF4 target genes with |log 2 FC| ≥ 1 and adjusted P < 0.01 are shown in red circles, and non-ATF4 target genes with |log 2 FC| ≥ 1 and adjusted P < 0.01 are shown in blue circles. ATF4 target genes with |log 2 FC| ≥ 2 and adjusted P < 0.01 are labeled. ( D ) Volcano plot showing differentially expressed SLCs with |log 2 FC| ≥ 1 and adjusted P < 0.01 labeled and shown in purple circles and other genes with |log 2 FC| ≥ 1 and adjusted P < 0.01 shown in green circles. ( E ) Expression levels of ATF4, <t>ATF3,</t> and CHOP evaluated in L363, MM.1S, KMS11, and KMS18 cells treated with 25 nM IACS for 24 hours by immunoblotting using actin as loading control.
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( A ) Key mitochondrial pathways down-regulated in GSEA performed in patients with MM from the CoMMpass trial (NCT0145429) with poor prognosis (PFS < 2 years, n = 426) versus better prognosis (PFS > 2 years, n = 341). Gene sets for shown pathways are derived from the Reactome database. FDR, false discovery rate. ( B ) Pathways up-regulated in GSEA of RNA-seq data from L363 cells treated with 25 nM IACS for 9 hours compared to vehicle control ( n = 3). KEGG, Kyoto Encyclopedia of Genes and Genomes. ( C ) Volcano plot showing differentially expressed ATF4 targets. ATF4 target genes with |log 2 FC| ≥ 1 and adjusted P < 0.01 are shown in red circles, and non-ATF4 target genes with |log 2 FC| ≥ 1 and adjusted P < 0.01 are shown in blue circles. ATF4 target genes with |log 2 FC| ≥ 2 and adjusted P < 0.01 are labeled. ( D ) Volcano plot showing differentially expressed SLCs with |log 2 FC| ≥ 1 and adjusted P < 0.01 labeled and shown in purple circles and other genes with |log 2 FC| ≥ 1 and adjusted P < 0.01 shown in green circles. ( E ) Expression levels of ATF4, <t>ATF3,</t> and CHOP evaluated in L363, MM.1S, KMS11, and KMS18 cells treated with 25 nM IACS for 24 hours by immunoblotting using actin as loading control.
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e coli  (ATCC)
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ATCC e coli
( A ) Key mitochondrial pathways down-regulated in GSEA performed in patients with MM from the CoMMpass trial (NCT0145429) with poor prognosis (PFS < 2 years, n = 426) versus better prognosis (PFS > 2 years, n = 341). Gene sets for shown pathways are derived from the Reactome database. FDR, false discovery rate. ( B ) Pathways up-regulated in GSEA of RNA-seq data from L363 cells treated with 25 nM IACS for 9 hours compared to vehicle control ( n = 3). KEGG, Kyoto Encyclopedia of Genes and Genomes. ( C ) Volcano plot showing differentially expressed ATF4 targets. ATF4 target genes with |log 2 FC| ≥ 1 and adjusted P < 0.01 are shown in red circles, and non-ATF4 target genes with |log 2 FC| ≥ 1 and adjusted P < 0.01 are shown in blue circles. ATF4 target genes with |log 2 FC| ≥ 2 and adjusted P < 0.01 are labeled. ( D ) Volcano plot showing differentially expressed SLCs with |log 2 FC| ≥ 1 and adjusted P < 0.01 labeled and shown in purple circles and other genes with |log 2 FC| ≥ 1 and adjusted P < 0.01 shown in green circles. ( E ) Expression levels of ATF4, <t>ATF3,</t> and CHOP evaluated in L363, MM.1S, KMS11, and KMS18 cells treated with 25 nM IACS for 24 hours by immunoblotting using actin as loading control.
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Image Search Results


( A ) Key mitochondrial pathways down-regulated in GSEA performed in patients with MM from the CoMMpass trial (NCT0145429) with poor prognosis (PFS < 2 years, n = 426) versus better prognosis (PFS > 2 years, n = 341). Gene sets for shown pathways are derived from the Reactome database. FDR, false discovery rate. ( B ) Pathways up-regulated in GSEA of RNA-seq data from L363 cells treated with 25 nM IACS for 9 hours compared to vehicle control ( n = 3). KEGG, Kyoto Encyclopedia of Genes and Genomes. ( C ) Volcano plot showing differentially expressed ATF4 targets. ATF4 target genes with |log 2 FC| ≥ 1 and adjusted P < 0.01 are shown in red circles, and non-ATF4 target genes with |log 2 FC| ≥ 1 and adjusted P < 0.01 are shown in blue circles. ATF4 target genes with |log 2 FC| ≥ 2 and adjusted P < 0.01 are labeled. ( D ) Volcano plot showing differentially expressed SLCs with |log 2 FC| ≥ 1 and adjusted P < 0.01 labeled and shown in purple circles and other genes with |log 2 FC| ≥ 1 and adjusted P < 0.01 shown in green circles. ( E ) Expression levels of ATF4, ATF3, and CHOP evaluated in L363, MM.1S, KMS11, and KMS18 cells treated with 25 nM IACS for 24 hours by immunoblotting using actin as loading control.

Journal: Science Advances

Article Title: Therapeutic implications of mitochondrial stress–induced proteasome inhibitor resistance in multiple myeloma

doi: 10.1126/sciadv.abq5575

Figure Lengend Snippet: ( A ) Key mitochondrial pathways down-regulated in GSEA performed in patients with MM from the CoMMpass trial (NCT0145429) with poor prognosis (PFS < 2 years, n = 426) versus better prognosis (PFS > 2 years, n = 341). Gene sets for shown pathways are derived from the Reactome database. FDR, false discovery rate. ( B ) Pathways up-regulated in GSEA of RNA-seq data from L363 cells treated with 25 nM IACS for 9 hours compared to vehicle control ( n = 3). KEGG, Kyoto Encyclopedia of Genes and Genomes. ( C ) Volcano plot showing differentially expressed ATF4 targets. ATF4 target genes with |log 2 FC| ≥ 1 and adjusted P < 0.01 are shown in red circles, and non-ATF4 target genes with |log 2 FC| ≥ 1 and adjusted P < 0.01 are shown in blue circles. ATF4 target genes with |log 2 FC| ≥ 2 and adjusted P < 0.01 are labeled. ( D ) Volcano plot showing differentially expressed SLCs with |log 2 FC| ≥ 1 and adjusted P < 0.01 labeled and shown in purple circles and other genes with |log 2 FC| ≥ 1 and adjusted P < 0.01 shown in green circles. ( E ) Expression levels of ATF4, ATF3, and CHOP evaluated in L363, MM.1S, KMS11, and KMS18 cells treated with 25 nM IACS for 24 hours by immunoblotting using actin as loading control.

Article Snippet: ATF4 (#11815), ATF3 (#33593), p-eIF2α (#3398), eIF2α (#2103), p-S6 (#2317), S6 (#4858), p-4EBP1 (#9456), 4EBP1 (#9644), p-EIF4E (#9741), EIF4E (9742S), p-AMPK (#2535S), AMPK (#2535), SESN2 (#8487), CHOP (#5554), ubiquitin (#3936), BCL-xL (#2764), BIM (#2933), PUMA (#4976), BAX (#2772), BID (#2002), BCL-2 (#4223), p-AMPKα (#2535), AMPKα (#2532), actin (#3700), and actin (#4970) were obtained from Cell Signaling Technology.

Techniques: Derivative Assay, RNA Sequencing, Control, Labeling, Expressing, Western Blot