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Image Search Results
Journal: Science Advances
Article Title: Therapeutic implications of mitochondrial stress–induced proteasome inhibitor resistance in multiple myeloma
doi: 10.1126/sciadv.abq5575
Figure Lengend Snippet: ( A ) Key mitochondrial pathways down-regulated in GSEA performed in patients with MM from the CoMMpass trial (NCT0145429) with poor prognosis (PFS < 2 years, n = 426) versus better prognosis (PFS > 2 years, n = 341). Gene sets for shown pathways are derived from the Reactome database. FDR, false discovery rate. ( B ) Pathways up-regulated in GSEA of RNA-seq data from L363 cells treated with 25 nM IACS for 9 hours compared to vehicle control ( n = 3). KEGG, Kyoto Encyclopedia of Genes and Genomes. ( C ) Volcano plot showing differentially expressed ATF4 targets. ATF4 target genes with |log 2 FC| ≥ 1 and adjusted P < 0.01 are shown in red circles, and non-ATF4 target genes with |log 2 FC| ≥ 1 and adjusted P < 0.01 are shown in blue circles. ATF4 target genes with |log 2 FC| ≥ 2 and adjusted P < 0.01 are labeled. ( D ) Volcano plot showing differentially expressed SLCs with |log 2 FC| ≥ 1 and adjusted P < 0.01 labeled and shown in purple circles and other genes with |log 2 FC| ≥ 1 and adjusted P < 0.01 shown in green circles. ( E ) Expression levels of ATF4, ATF3, and CHOP evaluated in L363, MM.1S, KMS11, and KMS18 cells treated with 25 nM IACS for 24 hours by immunoblotting using actin as loading control.
Article Snippet: ATF4 (#11815),
Techniques: Derivative Assay, RNA Sequencing, Control, Labeling, Expressing, Western Blot